David Giuntoli as Batman / Bruce Wayne (voice)

Patrick Fabian as Harvey Dent / Two-Face (voice)

John DiMaggio as James Gordon (voice)

Karan Brar as Sanjay 'Jay' Tawde (voice)

Navid Negahban as Ra's al Ghul (voice)

Darin De Paul as Thomas Wayne (voice)

Emily O'Brien as Talia al Ghul / Martha Wayne (voice)

Christopher Gorham as Oliver Queen / Green Arrow (voice)

David Dastmalchian as Grendon (voice)

Tim Russ as Lucius Fox (voice)

Matthew Waterson as Jason Blood / Etrigan (voice)

Jeffrey Combs as Kirk Langstrom (voice)

William Salyers as Cobblepot / Professor Manfurd (voice)

Gideon Adlon as Oracle / Poison Ivy (voice)

Tati Gabrielle as Kai Li Cain (voice)

Jason Marsden as Dick Grayson / Young Bruce Wayne (voice)

Brian George as Alfred Pennyworth (voice)