Frank Welker as Scooby-Doo / Fred Jones (voice)

Grey DeLisle as Daphne Blake / Wonder Woman (voice)

Matthew Lillard as Shaggy Rogers (voice)

Kate Micucci as Velma Dinkley (voice)

P.J. Byrne as J.B. (voice)

Victoria Grace as Mercy (voice)

Charles Halford as Lex Luthor (voice)

Nolan North as Computer / Joker / Superman (voice)

Tara Strong as Helen / Lois Lane / Harley Quinn (voice)

Fred Tatasciore as Solomon Grundy / Perry White (voice)

James Arnold Taylor as Jimmy Olsen / Rex Ruthor (voice)

Niccole Thurman as Mayor Flemming (voice)

Sam Vincent as Krypto (voice)

Brian Drummond as Streaky (voice)

Scott McNeil as Ace (voice)