Andy Samberg as Ham III (voice)

Cheryl Hines as Luna (voice)

Jeff Daniels as Zartog (voice)

Patrick Warburton as Titan (voice)

Kristin Chenoweth as Kilowatt (voice)

Kenan Thompson as Ringmaster (voice)

Zack Shada as Comet / Nava Boy (voice)

Carlos Alazraqui as Houston (voice)

Omid Abtahi as Dr. Jagu (voice)

Patrick Breen as Dr. Bob (voice)

Jane Lynch as Dr. Poole (voice)

Kath Soucie as Dr. Smothers (voice)

Stanley Tucci as Senator (voice)

Wally Wingert as Splork / Infinity Probe / Pappy Ham (voice)

Jessica Gee-George as Ship Voice / Infinity Probe / Additional Voices (voice)

Tom Kenny as Newsreel (voice)

Jason Harris as Guard (voice)

Jess Harnell as Additional Voices (voice)

Ellie Harvie as Additional Voices (voice)

Caitlin McKenna as Additional Voices (voice)