Clarence Nash as Donald Duck (voice)

Sterling Holloway as Professor Holloway (voice)

Joaquin Garay as Panchito (voice)

José Oliveira as José Carioca (voice)

Aurora Miranda as Brazilian Girl

Carmen Molina as Mexico Girl

Dora Luz as Mexico Girl

Frank Graham as (voice)

Fred Shields as (voice)

Nestor Amaral as (voice)

Jose de la Cruz Pacheco as (voice)

Jose Luis Garcia Rosales as (voice)

Luis Miranda Neri as (voice)

Emilia del Río as (voice)

Sara Ascencio as (voice)

Ofelia Ascencio as (voice)

Pinto Colvig as Aracuan Bird (voice) (uncredited)