Megumi Ogata as Shinji Ikari (voice)

Megumi Hayashibara as Rei Ayanami / Yui Ikari (voice)

Kotono Mitsuishi as Misato Katsuragi (voice)

Yuko Miyamura as Asuka Langley Soryu (voice)

Fumihiko Tachiki as Gendo Ikari (voice)

Miki Nagasawa as Maya Ibuki (voice)

Takehito Koyasu as Shigeru Aoba (voice)

Hiro Yuuki as Makoto Hyouga (voice)

Yuriko Yamaguchi as Ritsuko Akagi (voice)

Motomu Kiyokawa as Kozo Fuyutsuki (voice)

Akira Ishida as Kaworu Nagisa (voice)

Mugihito as Keel Lorenz (voice)

Koichi Yamadera as Ryoji Kaji (voice)

Tristan MacAvery as Gendo Ikari (English version) (voice)