Yoshirō Kitahara as Chief Inspector Wakabayashi

Junko Kano as Akiko Hayakawa

Ryūji Shinagawa as Dr. Tsukioka / The Invisible Man

Ikuko Mōri as Mieko

Jōji Tsurumi as Sugimoto

Yoshihiro Hamaguchi as Detective Hayama

Shōzō Nanbu as Dr. Hayakawa

Bontarō Miake as Chief of the Metropolitan Police

Ichirō Izawa as Kôkichi Kusunoki / The Human Fly II

Shizuo Chūjō as Yamada / The Human Fly I

Kô Sugita as Hajima

Tatsuo Hanabu as Director Tada

Fujio Harumoto as

Kōichi Itō as

Teppei Endō as Doctor

Gen Harada as

Jirō Tamiya as Plane Passenger (uncredited)