Millie Perkins as Anne Frank

Joseph Schildkraut as Otto Frank

Shelley Winters as Petronella Van Daan

Richard Beymer as Peter Van Daan

Gusti Huber as Edith Frank

Lou Jacobi as Hans Van Daan

Diane Baker as Margot Frank

Douglas Spencer as Kraler

Dodie Heath as Miep Gies

Ed Wynn as Albert Dussell

Robert Boon as SS Man (uncredited)

Delmar Erickson as SS Man (uncredited)

Gretchen Goertz as Sanne Devries (uncredited)

William Kirschner as Workman in Shop (uncredited)

Edmund Purdom as British Radio Announcer (voice) (uncredited)

Frank Tweddell as Night Watchman (uncredited)

Charles Wagenheim as Sneaky Thief (uncredited)