Ed Asner as J. Jonah Jameson (voice)

Dorian Harewood as Tombstone (voice)

Christopher Daniel Barnes as Spider-Man/Peter Parker (voice)

Sara Ballantine as Mary Jane Watson (voice)

Leigh-Allyn Baker as Alisha Silvermain (voice)

Maxwell Caulfield as Alistair Smythe (voice)

Linda Gary as Tante May Parker (voice)

Gary Imhoff as Harry Osborn (voice)

Roscoe Lee Browne as Kingpin/Wilson Fisk (voice)