Jodi Benson as Thumbelina (voice)

Gino Conforti as Jacquimo (voice)

Barbara Cook as Mother (voice)

Will Ryan as Hero / Reverend Rat (voice)

June Foray as Queen Tabitha (voice)

Kenneth Mars as King Colbert (voice)

Gary Imhoff as Prince Cornelius (voice)

Joe Lynch as Grundel (voice)

Charo as Mrs. Toad (voice)

Danny Mann as Mozo (voice)

Loren Lester as Gringo (voice)

Kendall Cunningham as Baby Bug (voice)

Tawny Sunshine Glover as Gnatty (voice)

Michael Nunes as Li'l Bee (voice)

Gilbert Gottfried as Berkeley Beetle (voice)

Pat Musick as Mrs Rabbit (voice)

Neil Ross as Mr. Fox / Mr. Bear (voice)

Carol Channing as Ms. Fieldmouse (voice)

John Hurt as Mr. Mole (voice)

Tony Jay as Cow (voice)

Randy Crenshaw as Berkeley Beetle (singing voice)

Stevie Vallance as (voice)