Peter Cushing as Baron Victor Frankenstein

Veronica Carlson as Anna Spengler

Freddie Jones as Prof. Richter

Simon Ward as Dr. Karl Holst

Thorley Walters as Insp. Fritsch

Maxine Audley as Ella Brandt

George Pravda as Dr. Frederick Brandt

Geoffrey Bayldon as Police Doctor

Colette O'Neil as Mad Woman

Frank Middlemass as Guest - Plumber

George Belbin as Guest - Playing chess

Norman Shelley as Guest - Smoking pipe

Michael Gover as Guest - Reading newspaper

Peter Copley as Principal

Jim Collier as Dr. Heidecke

Allan Surtees as Police Sergeant

Windsor Davies as Police Sergeant

Harry Fielder as Villager (uncredited)

Caron Gardner as Passer-by (uncredited)

Victor Harrington as Journalist (uncredited)

Harold Goodwin as Burglar (uncredited)

Timothy Davies as Policeman (uncredited)

Jack Armstrong as Reporter (uncredited)

Elizabeth Morgan as Christina (uncredited)

Dorothy Smith as Anna's Neighbour (uncredited)

Pauline Chamberlain as Woman in Street (uncredited)

Ernest Fennemore as Policeman (uncredited)

Robert Gillespie as Mortuary Attendant (uncredited)

Michael Goldie as Warder (uncredited)

Arthur Howell as Body in Glass Case (uncredited)

Daphne Oxenford as Lady In Garden (uncredited)