Gene Autry as Gene Autry

Champion as Gene's Horse

Gail Davis as Dell Middler

Jim Davis as Wade McQuarrie

Bob Steele as Walt Middler

Edgar Dearing as Colonel Middler

Richard Alexander as Henchman Luke Anders

Terry Frost as Henchman Wyatt

Peter Mamakos as Laughing Jack

Steve Clark as Dr. Seddon

Stanley Andrews as Major Weatherly

Duke York as Sergeant Laughlin

Eugene Borden as Gus Poppalaardo

Pat Buttram as Pat

Stanley Andrews as Major Weatherly (uncredited)

Bobby Clack as Cowhand (uncredited)