Debi Derryberry as Jimmy Neutron (voice)

Tara Strong as Timmy Turner (voice)

Jeff Garlin as Villain (voice)

Rob Paulsen as Carl / Eustace / Butler / Bucky McBadbat (voice)

Jeffrey Garcia as Sheen Estevez (voice)

Carolyn Lawrence as Cindy Vortex / Mrs. Folfax (voice)

Crystal Scales as Libby Folfax (voice)

Mark DeCarlo as Hugh Neutron (voice)

Megan Cavanagh as Judy Neutron (voice)

Jason Marsden as Chester McBadbat (voice)

Gary LeRoi Gray as A.J. (voice)

Susanne Blakeslee as Wanda / Mom (voice)

Daran Norris as Cosmo / Dad (voice)

Carlos Alazraqui as Mr. Crocker (voice)

Billy West as Blix / British Official / Sam / Corky (voice)

Jay Leno as Nega-Chin (voice)

Grey DeLisle as Vicky (voice)