Yo Oizumi as Professor Layton (voice)

Maki Horikita as Luke Triton (voice)

Saki Aibu as Emmy Altava (voice)

Nana Mizuki as Janice Quatlane (voice)

Atsuro Watabe as Jean Descole (voice)

Hochu Otsuka as Inspector Clamp Grosky (voice)

Iemasa Kayumi as Oswald Whistler (voice)

Fumiko Orikasa as Melina Whistler (voice)

Kikuko Inoue as Celia Raidley (voice)

Sumire Morohoshi as Nina (voice)

Shozo Iizuka as Curtis O'Donnell (voice)

Kenta Miyake as Marco Brock (voice)

Megumi Toyoguchi as Amelia Ruth (voice)

Jouji Nakata as Frederick Bargland (voice)

LiLiCo as Annie Dretche (voice)

Koichi Yamadera as Pierre Starbuck (voice)

Minoru Inaba as Don Paolo (voice)

Shirou Saitou as Inspector Chelmey (voice)

Mamiko Noto as Flora Reinhold (voice)

Rokuro Naya as Dr. Andrew Schrader (voice)