Mae Whitman as Barbara Gordon/Batgirl (voice)

Anais Fairweather as Supergirl / Kara Zor-El (voice)

Grey DeLisle as Wonder Woman / Platinum (voice)

Teala Dunn as Bumblebee / Karen Beecher / Artemiz (voice)

Tara Strong as Harley Quinn/ Harleen Quinzel / Poison Ivy / Pamela Isley (voice)

Phil LaMarr as Doc Magnus (voice)

John DiMaggio as Ambassador Bek (voice)

Jessica DiCicco as Star Sapphire / Carol Ferris / Lashina (voice)

Yvette Nicole Brown as Principal Waller (voice)

Nika Futterman as Hawkgirl (voice)

Tania Gunadi as Lady Shiva (voice)

Tom Kenny as Sinestro / Lobo (voice)

Danica McKellar as Frost (voice)

Fred Tatasciore as Brainia / Kryptomites (voice)

Hynden Walch as Starfire / Koriand'r/ Blackfire (voice)

Alexis G. Zall as Lois Lane (voice)