James Corden as Peter Rabbit (voice)

Rose Byrne as Bea

Domhnall Gleeson as Mr. Thomas McGregor

David Oyelowo as Nigel Basil-Jones

Margot Robbie as Flopsy Rabbit / The Narrator (voice)

Elizabeth Debicki as Mopsy Rabbit (voice)

Aimee Horne as Cottontail Rabbit (voice)

Colin Moody as Benjamin Bunny (voice)

Lennie James as Barnabas (voice)

Damon Herriman as Tom Kitten (voice)

Hayley Atwell as Mittens (voice)

Rupert Degas as Samuel Whiskers / Little Pig Robinson (voice)

Sia as Mrs. Tiggy-Winkle (voice)

Sam Neill as Tommy Brock (voice)

Ewen Leslie as Pigling Bland (voice)

David Wenham as Johnny Town-Mouse (voice)

Will Reichelt as JW Rooster II (voice)

Christian Gazal as Felix D'eer (voice)

Tim Minchin as Busker K. Bushy, Esq (voice)

Stewart Alves as Mr. Tod (voice)

Faizan Munawar Varya as Ray Rabbit (voice)

Tara Morice as Officiant

Dave Lawson as Chris

Alex Blias as Phil

Jude Hyland as Toy Shop Young Boy

Neil Hayes as Toy Shop Father

Neilesh Ambu as Passerby (uncredited)