Keanu Reeves as Neo ("Kid's Story") (voice)

Carrie-Anne Moss as Trinity ("Kid's Story" and "A Detective Story") (voice)

Clayton Watson as The Kid ("Kid's Story") (voice)

Pamela Adlon as Jue ("Final Flight of the Osiris") / Manabu ("Beyond") (voice)

Melinda Clarke as Alexa ("Matriculated") (voice)

John DiMaggio as Crew Man ("Final Flight of the Osiris") / Kaiser ("Program") (voice)

Kevin Michael Richardson as Thadeus ("Final Flight of Osiris") / Cop ("Kid's Story") / Agent #2 ("World Record")

Tom Kenny as Operator ("Final Flight of Osiris")

Rick Gomez as Pilot ("Final Flight of Osiris")

Tara Strong as Crew Woman ("Final Flight of Osiris") / Nurse ("World Record") / Misha ("Beyond")

Bette Ford as Old Woman ("Final Flight of Osiris")

Julia Fletcher as The Instructor ("The Second Renaissance Part I & II") / Narrator ("World Record")

Dane A. Davis as 01 Versatran Spokesman ("The Second Renaissance Part I & II")

Debi Derryberry as Kid ("The Second Renaissance Part I & II")

Jill Talley as Mother ("The Second Renaissance Part I & II")

Dwight Schultz as Additional Voices ("The Second Renaissance Part I & II") / Monaka ("Matriculated")

James Arnold Taylor as Additional Voices ("The Second Renaissance Part I & II") / Ash ("A Detective Story") / Raul ("Matriculated")

John DeMita as Teacher ("Kid's Story")

Hedy Burress as Cis ("Program") / Yoko ("Beyond")

Phil LaMarr as Duo ("Program")

Victor Williams as Dan ("World Record")

John Wesley as Dan's Dad ("World Record")

Alex Fernandez as Tom ("World Record")

Allison Smith as Reporter ("World Record")

Matt McKenzie as Agent #1 ("World Record") / Agent ("Beyond") / Agent ("A Detective Story")

Tress MacNeille as Housewife ("Beyond") / Kenny ("Beyond")

Kath Soucie as Pudgy ("Beyond") / Masa ("Beyond") / Sara ("Beyond")

Terrence 'T.C.' Carson as Clarence ("A Detective Story")

Rodney Saulsberry as Chyron ("Matriculated")

Olivia d'Abo as Rox ("Matriculated")

Jack Fletcher as Sandro ("Matriculated")

Akio Otsuka as Thadeus (voice)