Paul Reubens as Pee-wee Herman

E. G. Daily as Dottie

Mark Holton as Francis

Diane Salinger as Simone

Judd Omen as Mickey

Irving Hellman as Mr. Crowtrey

Monte Landis as Mario

Damon Martin as Chip

Alice Nunn as Large Marge

David C. Glasser as BMX Kid

Gregory Brown as BMX Kid

Daryl Keith Roach as Chuck

Bill Cable as Policeman #1

Peter Looney as Policeman #2

Starletta DuPois as Sgt. Hunter

Professor Toru Tanaka as Butler

Ed Herlihy as Mr. Buxton

Ralph Seymour as Francis' Accomplice

Lou Cutell as Amazing Larry

Raymond Martino as Gang Member

Erica Yohn as Madam Ruby

Bill W. Richmond as Highway Patrolman

Ed Griffith as Trucker

Simmy Bow as Man in Diner

Jon Harris as Andy

Carmen Filpi as Hobo Jack

Jan Hooks as Tina

John Moody as Bus Clerk

John O'Neill as Cowboy #1

Alex Sharp as Cowboy #2

Chester Grimes as Biker #1

Luis Contreras as Biker #2

Lonnie Parkinson as Biker #3

Howard Hirdler as Biker #4

Cassandra Peterson as Biker Mama

Jason Hervey as Kevin Morton

Bob McClurg as Studio Guard

John Paragon as Movie Lot Actor

Susan Barnes as Movie Lot Actress

Zachary Hoffman as Director

Lynne Marie Stewart as Mother Superior

George Sasaki as Japanese Director

Richard Brose as Tarzan

Drew Seward as Kid #1

Brett Fellman as Kid #2

Bob Drew as Fireman

John Gilgreen as Policeman at Pet Shop

Noreen Hennessey as Reporter

Phil Hartman as Reporter

Michael Varhol as Photographer

David Rothenberg as Hobo

Patrick Cranshaw as Hobo

Sunshine Parker as Hobo

Gilles Savard as Pierre

James Brolin as "P. W."

Morgan Fairchild as "Dottie"

Tony Bill as Terry Hawthorne

Dee Snider as Self

Milton Berle as Self (uncredited)

Terry Bolo as Biker Chick (uncredited)

Tim Burton as Thug in Alley (uncredited)

Manuel Benitez as BMX Kid #0