Keanu Reeves as Kai

Hiroyuki Sanada as Kuranosuke Ôishi

Ko Shibasaki as Mika

Tadanobu Asano as Lord Kira

Min Tanaka as Lord Asano

Rinko Kikuchi as Witch

Jin Akanishi as Chikara

Masayoshi Haneda as Yasuno

Hiroshi Sogabe as Hazama

Takato Yonemoto as Basho

Shû Nakajima as Horibe

Hiroshi Yamada as Hara

Cary-Hiroyuki Tagawa as Shogun Tsunayoshi

Tanroh Ishida as Shogun's Adjutant

Yorick van Wageningen as Kapitan

Ron Bottitta as Narrator

Natsuki Kunimoto as Riku

Togo Igawa as Tengu Lord

Akira Koieyama as Kira's Spy

Haruka Abe as Mika's Handmaiden

Clyde Kusatsu as Drunk Official

Junichi Kajioka as Guard

Masashi Fujimoto as Lead Soldier

Neil Fingleton as Lovecraftian Samurai

Rick Genest as Foreman

Masayuki Deai as Isogai