Johnny Knoxville as Luke Duke

Seann William Scott as Bo Duke

Jessica Simpson as Daisy Duke

Willie Nelson as Uncle Jesse Duke

Burt Reynolds as Jefferson Davis 'Boss' Hogg

M.C. Gainey as Sheriff Rosco P. Coltrane

James Roday Rodriguez as Billy Prickett

David Koechner as Cooter Davenport

Michael Weston as Deputy Enos Strate

Nikki Griffin as Katie Johnson

Alice Greczyn as Laurie Pullman

Joe Don Baker as Governor Jim Applewhite

Lynda Carter as Pauline

Kevin Heffernan as Sheev

Steve Lemme as Jimmy

Michael Roof as Dil Driscoll

Jack Polick as Deputy Cletus Hogg

Jacqui Maxwell as Annette

Charlie Finn as Royce

Therial 'Houseman' DeClouet as Judge Locke Randolph

Jim Cody Williams as Security Guard Chip

Wayne Douglas Morgan as Prisoner

Junior Brown as The Balladeer (voice)

Tobi Gadison as

Andrew Prine as Angry Man

David Leitch as Puncher

Christina Dare as Sorority Girl

Jay Chandrasekhar as Campus Cop #1

Erik Stolhanske as Campus Cop #2

A.J. Foyt IV as Race Car Driver #1

Dolan Wilson as Local #2

Heather Hemmens as Girl #1

Mitch Braswell as Out of Towner #1

Rusty Tennant as Local #1