Warner Baxter as Dr. Samuel Alexander Mudd

Gloria Stuart as Mrs. Peggy Mudd

Claude Gillingwater as Colonel Dyer

Arthur Byron as Mr. Erickson

O. P. Heggie as Dr. Mac Intyre

Harry Carey as Commandant

Francis Ford as Corporal O'Toole

John McGuire as Lieutenant Lovett

Francis McDonald as John Wilkes Booth

Douglas Wood as General Ewing

John Carradine as Sergeant Rankin

Joyce Kay as Martha Mudd

Fred Kohler Jr. as Sergeant Cooper

Ernest Whitman as Buck

Paul Fix as David Herold

Frank Shannon as Mr. Holt

Frank McGlynn Sr. as Abraham Lincoln

Leila McIntyre as Mrs. Abraham Lincoln

Etta McDaniel as Aunt Rosabelle

J.M. Kerrigan as Judge Maiben

Arthur Loft as Carpet Bagger

Paul McVey as General Hunter

Maurice Murphy as Orderly

Matthew Beard as Boy at Dr. Mudd's Door (uncredited)

Frank Baker as Undetermined Secondary Role (uncredited)

Stanley Blystone as Boy Seeking Dr. Mudd (uncredited)

Stanley Bordagaray as Undetermined Secondary Role (uncredited)

Whitney Bourne as Undetermined Secondary Role (uncredited)

Lester Dorr as George A. Atzerodt (uncredited)

Robert Dudley as Druggist at Trial (uncredited)

Jan Duggan as Actress at Ford's Theatre (uncredited)

Earl Eby as Usher (uncredited)

Dick Elliott as Actor at Ford's Theatre (uncredited)

Bess Flowers as Woman Sitting Behind Lincoln in Theatre Box (uncredited)

Bud Geary as Sergeant (uncredited)

Melvin J. Gibby as Lewis Payne (uncredited)

Charles Haefeli as Prisoner (uncredited)

Robert Homans as Sergeant (uncredited)

John Lester Johnson as Black Soldier at Prison (uncredited)

Beulah Hall Jones as Blanche (uncredited)

Paul Kruger as Soldier (uncredited)

Duke R. Lee as Sergeant (uncredited)

Wilfred Lucas as Testifying Colonel (uncredited)

Murdock MacQuarrie as Edman Spangler (uncredited)

James A. Marcus as Blacksmith (uncredited)

Eric Mayne as Court-Martial Participant (uncredited)

Paul McAllister as Doctor (uncredited)

Merrill McCormick as Commandant's Aide (uncredited)

J.P. McGowan as Ship's Captain (uncredited)

Arthur Millett as Undetermined Secondary Role (uncredited)

Wedgwood Nowell as Court-Martial Member (uncredited)

Robert Parrish as Undetermined Secondary Role (uncredited)

Vester Pegg as Soldier (uncredited)

Jack Pennick as Corporal (uncredited)

George H. Reed as Black Man Giving Booth Directions (uncredited)

Paul Stanton as Agitating Orator (uncredited)

Tom Steele as Trooper (uncredited)

Harry Strang as Ship's Mate (uncredited)

Cyril Thornton as Michael O'Laughlin (uncredited)

Ray Turner as Black Soldier at Prison (uncredited)

Blue Washington as Black Soldier at Prison (uncredited)

Charles West as Washington (DC) Citizen (uncredited)

Cecil Weston as Mary Surratt (uncredited)

Lloyd Whitlock as Maj. Rathbone (uncredited)