Vera Drew as Joker the Harlequin

Lynn Downey as Joker's Mom

Kane Distler as Mr. J

Nathan Faustyn as Penguin

David Liebe Hart as Ra's Al Ghul

Phil Braun as Batsy (voice)

Griffin Kramer as Young Joker

Christian Calloway as Doctor Crane

Trevor Drinkwater as Edward Nygma aka The Riddler

Ruin Carroll as Ivy (voice)

Tim Heidecker as Perry White (voice)

Denali Winter as Clark

Bambi Belle as Lois

Ember Knight as Mxy (voice)

Bob Odenkirk as Bob the Goon (voice)

Scott Aukerman as Mister Frieze (voice)

Alec Robbins as Mr. Boop

Sandy Honig as Mr. Boop’s Wife

Dan Curry as Bane

Cassie Hamilton as Nicole Kidman

Jay Speedy Weingarten as The Clown Who Gags

Matthew Goldin as Stiles Stilinski

Eliot Glazer as Lil Timmy Too Times

Cricket Arrison as 'Creeper'

Mia Moore Marchant as Helena the Huntress

Maria Bamford as Lorne Michaels (voice)

Robert Wuhl as Robert Wuhl

Austen Reeder as Smylex Narrator

Abby Clark as Sad Wife

Benjamin Morey as Golfing Husband

Dan Kypers as UCB Orientation Narrator / Mad Hatter

Jeremy Franchi as Suicide Cop

Ted Reidy as Jimmy Olsen

Daniella Baker as Catwoman

Lydia Wolfie as Anarky / Harlequin Auditioner

David Allen as Suicide Cop's Partner

Nateo Caballero as UCB Receptionist

Arden Hughes as Audition System (voice)

Harry McDonough as UCB MC / Det. Harvey Bullock

Rome Davis as Therapist

Jeremy Long as Det. Melody McKenna

Elanor Cassady as Hotline Operator

Susan&Denise as Serve the Date Ladies

Beatrice DiCicco as Newscaster 1

Emily Lynch as Newscaster 2 (voice)

Michelle Stone as Newscaster 3

Ashe Pignataro as Newscaster 4

Calvin DiLibero as Warmup Jokeman

Zoe Limbrick as Warmup Harlequin

Jack Walker Nixon as Ledger Jokeman

Isabella Marino as Dini Harlequin 1

Wylie Anderson as Dini Harlequin 2

Jake Noll as Freddy