Dakota Fanning as Fern Arable

Julia Roberts as Charlotte the Spider (voice)

Steve Buscemi as Templeton the Rat (voice)

John Cleese as Samuel the Sheep (voice)

Oprah Winfrey as Gussy the Goose (voice)

Cedric the Entertainer as Golly the Goose (voice)

Reba McEntire as Betsy the Cow (voice)

Kathy Bates as Bitsy the Cow (voice)

Robert Redford as Ike the Horse (voice)

Thomas Haden Church as Brooks the Crow (voice)

André 3000 as Elwyn the Crow (voice)

Dominic Scott Kay as Wilbur (voice)

Sam Shepard as Narrator (voice)

Abraham Benrubi as Uncle the Pig (voice)

Kevin Anderson as Mr. Arable

Essie Davis as Mrs. Arable

Siobhan Fallon Hogan as Mrs. Zuckerman

Louis Corbett as Avery

Robyn Arthur as Teacher

Julian O'Donnell as Henry Fussy

Gary Basaraba as Homer Zuckerman

Nate Mooney as Lurvy

Nicholas Bell as Minister

Beau Bridges as Dr. Dorian

Teague Rook as Well Dressed Man

Julia Zemiro as Bystander

Denise Kirby as New Teacher

Robert Plazek as Ball Thrower

Joseph Lotesto as Young Boy

Michael Roland as Mayor

Don Bridges as Announcer

Ian Watkin as Fair Official

Joel McCrary as Sheep Group (voice)

Brian Stepanek as Sheep Group (voice)

Fred Tatasciore as Sheep Group (voice)

Bradley White as Sheep Group (voice)

Maia Kirkpatrick as Joy the Baby Spider (voice)

Jennessa Rose as Aranea the Baby Spider (voice)

Briana Hodge as Nellie the Baby Spider (voice)

Dale Azzopardi as Photographer (uncredited)

Ella Scott Lynch as Girl at Fair (uncredited)