Michael Fassbender as Callum Lynch / Aguilar de Nerha

Marion Cotillard as Dr. Sophia Rikkin

Jeremy Irons as Alan Rikkin

Brendan Gleeson as Joseph Lynch

Charlotte Rampling as Ellen Kaye

Michael Kenneth Williams as Moussa

Ariane Labed as Maria

James Sobol Kelly as Father Raymond

Denis Ménochet as Abstergo Güvenlik Müdürü

Callum Turner as Nathan

Khalid Abdalla as Sultan Muhammad XII

Essie Davis as Mary Lynch

Carlos Bardem as Benedicto

Javier Gutiérrez as Tomas de Torquemada

Hovik Keuchkerian as Ojeda

Crystal Clarke as Samia

Brian Gleeson as Young Joseph

Rufus Wright as Alex

Matias Varela as Emir

Coco König as Orderly

Michelle H. Lin as Lin

Julio Jordán as General Ramirez

Angus Brown as Young Cal

Kemaal Deen-Ellis as Prince Ahmed

Aaron Monaghan as Gilles

Thomas Camilleri as King Ferdinand

Marysia S. Peres as Queen Isabella

Gabriel Andreu as Christopher Columbus

Jeff Mash as Prison Warden

Yuric Allison as Muhammad's Official

Joe Kennard as Abstergo Guard

Gertrude Thoma as Speaker

Octavia Selena Alexandru as Lara (uncredited)

Iain Batchelor as Abstergo Guard 1 (uncredited)

Dacio Caballero as Assasin 3 (uncredited)

Emma Clay as Child Chorister (uncredited)

Graham Curry as Templar (uncredited)

Jem Kai Olsen as Templar (uncredited)

Louis Dulanto-Sime as Chorister (uncredited)

Malcolm Ellul as Executioner (uncredited)

Dino Fazzani as Prison Guard (uncredited)

Lee Nicholas Harris as Paramedic (uncredited)

Bruce Lester-Johnson as Prison Guard (uncredited)

Mohammed Ali as Infinity Patient

Jade Clarke as Prisoner

Alexander Cooper as Passerby

Ty Hurley as Freemason

Matthew Leonhart as Templar

Simon Manley as Guard

Juan Carlos Martínez Antuña as Templar

Martyn Mayger as Templar

Stephen McDade as Templar

Anthony Milton as Templar Guard (uncredited)

Ross Moneypenny as Templar

Jassie Mortimer as Female Mason

Cali Nelle as Assassin #2

Emeson Nwolie as Administration Officer

Massimo Portelli as Spanish Crossbowman

Jonathan Pyatt as Spanish Guard

Mike Ray as Templar Guest

Jonathan Ronin as Spanish Guard

Steve Saunders as Patient

Jasper Schellekens as Spanish Infantry

Rahji Shrinarine as Templar

Clem So as Animus Patient

Clyde Vassallo as Spanish Infantry

Sean Vassallo as Spanish Archer

Roberto Vivancos as Merchant

Roberto Vivancos as Merchant

Abi Whetton as Choir Member

Christopher Whitlow as Assassin #1

Cerys Wrigley-Moss as Chorister

Juan Pablo Shuk as Father

Maxime Durand as Spanish Archer (uncredited)