James Arnold Taylor as Leonardo (voice)

Mitchell Whitfield as Donatello / Additional Voices (voice)

Nolan North as Raphael / Nightwatcher (voice)

Mikey Kelley as Michelangelo (voice)

Sarah Michelle Gellar as April O'Neil (voice)

Chris Evans as Casey Jones (voice)

Mako as Splinter (voice)

Patrick Stewart as Winters (voice)

Kevin Smith as Diner Cook (voice)

Laurence Fishburne as Narrator (voice)

Zhang Ziyi as Karai (voice)

John DiMaggio as Colonel Santino (voice)

Paula Mattioli as General Serpiente (voice)

Kevin Michael Richardson as General Aguila / Additional Voices (voice)

Fred Tatasciore as General Gato / Additional Voices (voice)

Dee Bradley Baker as Additional Voices (voice)

Greg Baldwin as Splinter (voice)

Jeff Bennett as Additional Voices (voice)

Jim Cummings as Additional Voices (voice)

Grey DeLisle as Additional Voices (voice)

Chris Edgerly as Additional Voices (voice)

Kim Mai Guest as Additional Voices (voice)

Jennifer Hale as Additional Voices (voice)

Jess Harnell as Additional Voices (voice)

Phil LaMarr as Additional Voices (voice)

Paul Michael Robinson as Additional Voices (voice)

Tara Strong as Additional Voices (voice)

Billy West as Additional Voices (voice)

Kevin Munroe as Diner Patron (voice) (uncredited)