Josh Flitter as Ace Ventura, Jr.

Emma Lockhart as Laura Wilson

Ann Cusack as Melissa Ventura

Cullen Douglas as Dr. Sickinger

Art LaFleur as Russell Hollander

Austin Rogers as A Plus

Reed Alexander as Quenton Pennington, Jr.

Carina Conti as Tara

Ralph Waite as Grandpa Ventura

Ashley Milchman as Emo Kid #1

Brooke Milchman as Emo Kid #2

Aubrey Shea as Daniella

Morgan Curschman as Girl Swimmer #1

Paige Huston as Girl Swimmer #2

Lisa Glaze as Teacher

Kendall Ganey as Little Girl at Spelling Bee

Tom Hillmann as Trooper

Trevin Lica as Bully

Jabari Ty'ree Shaffer as Boy

Jeff Chase as Pennington's Bodyguard

John Connon as Tour Guide

Veryl Jones as Museum Security Guard

Phil Ramirez as Prosecutor

Jim R. Coleman as Judge

Karel Wright as Tabby's Handler

Jeff Farley as Tabby's Security Officer

William Haze as Magician

Terry Loughlin as Horse Owner

Patrick Hoover as Party Kid #1

Haley Sehgal as Party Kid #2

Connor Boyle as Overwhelmed Kid

Brian Patrick Clarke as Quenton Pennington Sr.

Adam Vernier as Agent #1

Tom Brooks as Agent #2

Shane Costa as Pennington's Bodyguard

Cristina Huang as Park Guest

Dakota Marooney as Kid at Party

Brandon Migliore as Crash Car Driver

Rhiannon Roberts as Park Guest

Chris Adler as Pennington's Bodyguard (uncredited)

Karl Anthony as Laura's Dad (uncredited)

Jennifer Badger as Security Team Leader (uncredited)

Sam Bauso as FBI Agent (uncredited)

Jessica Blackmore as Assistant with White Horse (uncredited)

Lisa Buckley as Miss Foster - Ox's Pet Store Clerk (uncredited)

Chase "Uzzle" Carter as FBI Agent (uncredited)

Omar Elkalyoubie as Chinese Dignitary (uncredited)

Bayley Ellenburg as Witness (uncredited)

Emily Rose Everhard as Additional Voices (voice) (uncredited)

Jen Halbert as Tabby's Entourage (uncredited)

Annie Kidwell as County Prisoner (uncredited)

Jesse Kozel as Neighbor (uncredited)

Noah Kronenberger as Park Guest (uncredited)

Jennifer Lonardo as Teacher (uncredited)

Sam Lonigro as Girls Swim Team Member (uncredited)

Leslie Maine as FBI Agent (uncredited)

Jeremy Palko as CSI Agent (uncredited)

Noah Schnacky as Swim Team Boy (uncredited)

David W. Scott as Pennington's Bodyguard (uncredited)

Andy Ussach as Pennington's Butler (uncredited)

Justin Brown as Churro Guy (uncredited)