Rex Harrison as Dr. John Dolittle

Samantha Eggar as Emma Fairfax

Anthony Newley as Matthew Mugg

Richard Attenborough as Albert Blossom

Peter Bull as General Bellowes

Muriel Landers as Mrs. Blossom

William Dix as Tommy Stubbins

Geoffrey Holder as William Shakespeare X

Portia Nelson as Sarah Dolittle

Norma Varden as Lady Fetherington

Frank Baker as Trial Spectator (uncredited)

Edward Cast as Prison Guard (uncredited)

Phyllis Coghlan as Courtroom Spectator (uncredited)

Robert Cole as Roustabout (uncredited)

Cyril Cross as Charlie (uncredited)

Peter Crowcroft as Assistant Judge (uncredited)

John Dolan as Fisherman (uncredited)

Jesslyn Fax as Old Woman (uncredited)

Arthur Gould-Porter as Sir Rupert (uncredited)

Eric Heath as Courtroom Spectator (uncredited)

Lars Hensen as Minor Role (uncredited)

Kendrick Huxham as Elderly Man (uncredited)

Theron Jackson as Boy (uncredited)

Queenie Leonard as Courtroom Spectator (uncredited)

Frank Radcliffe as Strong Man (uncredited)

Jack Raine as Vicar (uncredited)

Danny Rees as Juggler (uncredited)

Angelo Rossitto as Dwarf (uncredited)

Rufus as Dog (uncredited)

Ted Stanhope as Assistant Judge (uncredited)

Geoffrey Steele as Courtroom Spectator (uncredited)

Gilchrist Stuart as The Vicar (uncredited)

Ginny Tyler as Polynesia (voice) (uncredited)

Bob Winters as Juggler (uncredited)

Judy Chapman as Dancer (uncredited)