Tim Daly as Superman / Clark Kent (voice)

Dana Delany as Lois Lane (voice)

Kevin Conroy as Batman / Bruce Wayne (voice)

Clancy Brown as Lex Luthor (voice)

Mark Hamill as The Joker (voice)

Arleen Sorkin as Harley Quinn (voice)

Lisa Edelstein as Mercy Graves (voice)

Bob Hastings as Commissioner Gordon (voice)

Efrem Zimbalist Jr. as Alfred (voice)

Joseph Bologna as Dan Turpin (voice)

Robert Costanzo as Detective Bullock (voice)

George Dzundza as Perry White (voice)

Lauren Tom as Angela Chen (voice)

Brad Garrett as Bibbo (voice) (uncredited)