Roger Craig Smith as Batman (voice)

Oded Fehr as Mr. Freeze (voice)

Lucien Dodge as Robin (voice)

Dana Snyder as The Penguin (voice)

Chris Diamantopoulos as Green Arrow (voice)

Phil LaMarr as Dr. Kirk Langstrom (voice)

Will Friedle as Nightwing (voice)

Charlie Schlatter as The Flash (voice)

Carlos Alazraqui as Bane (voice)

John DiMaggio as Killer Croc (voice)

Dave B. Mitchell as Clayface (voice)

Troy Baker as The Joker (voice)

Alastair Duncan as Alfred Pennyworth (voice)

Janell Cox as Newscaster / Old Woman (voice)

Richard Epcar as Commissioner James Gordon (voice)