Basil Rathbone as Sherlock Holmes

Nigel Bruce as Dr. Watson

Ida Lupino as Ann Brandon

Alan Marshal as Jerrold Hunter

Terry Kilburn as Billy

George Zucco as Professor Moriarty

Henry Stephenson as Sir Ronald Ramsgate

E. E. Clive as Inspector Bristol

Arthur Hohl as Henchman Bassick

May Beatty as Mrs. Jameson, Brandon Housekeeper

Peter Willes as Lloyd Brandon

Mary Gordon as Mrs. Hudson

Holmes Herbert as Justice of the Court

George Regas as Henchman Mateo

Mary Forbes as Lady Conyngham

Frank Dawson as Dawes, Moriarty's servant

William Austin as Inquisitive Stranger

Anthony Kemble-Cooper as Tony Conyngham

Leonard Mudie as Barrows - Moriarty's Attorney (uncredited)

Robert Nobel as Foreman of the Jury (uncredited)

Ivan F. Simpson as Gates - Trial Prosecutor (uncredited)

Neil Fitzgerald as Clerk of the Court (uncredited)

Frank Baker as Tompkins (uncredited)

Denis Greene as Denis Green (uncredited)

Robert Cory as Tower Sentry (uncredited)

Gordon Hart as Guard (uncredited)

Keith Hitchcock as Constable (uncredited)

Ivo Henderson as Bobby (uncredited)

Leyland Hodgson as Bobby (uncredited)

Boyd Irwin as Bobby (uncredited)

Charles Irwin as Marine Sergeant (uncredited)

Robert R. Stephenson as Cabby (uncredited)

Harry Cording as Second Fake Constable (uncredited)

Eric Wilton as Conyngham Butler (uncredited)

Frank Benson as Cockney (uncredited)

Ted Billings as Pub Customer (uncredited)

Herbert Evans as Scotland Yard Man (uncredited)