Richard Greene as Sir Henry Baskerville

Basil Rathbone as Sherlock Holmes

Wendy Barrie as Beryl Stapleton

Nigel Bruce as Doctor John H. Watson

Lionel Atwill as Dr. James Mortimer

John Carradine as Barryman

Barlowe Borland as Frankland

Beryl Mercer as Mrs. Jennifer Mortimer

Morton Lowry as John 'Jack' Stapleton

Ralph Forbes as Sir Hugo Baskerville

E. E. Clive as London Cabbie John Clayton

Eily Malyon as Mrs. Barryman

Lionel Pape as The Coroner

Nigel De Brulier as The Notting Hill Murderer

Mary Gordon as Mrs. Hudson

Ian Maclaren as Sir Charles Baskerville

Ruth Terry as Betsy Ann

Mary Young as Betsy Ann's Mother (uncredited)

Kenneth Hunter as Ship's Officer (uncredited)

Vesey O'Davoren as Ship's Steward (uncredited)

John Graham Spacey as Ship's Porter (uncredited)

David Thursby as Open Carriage Driver (uncredited)

Peter Willes as Roderick

Leonard Carey as Hugo's Servant (uncredited)

Rita Page as Chambermaid (uncredited)

Jack Egger as (uncredited)

Frank McLure as Ship Passenger (uncredited)

Brandon Beach as Ship Passenger (uncredited)

Blitzen as The Hound (as Chief)

James Carlisle as Ship Passenger (uncredited)

Herschel Graham as Ship Passenger (uncredited)