Edward G. Robinson as Dr. T.S. Clitterhouse

Claire Trevor as Jo Keller

Humphrey Bogart as 'Rocks' Valentine

Allen Jenkins as Okay

Donald Crisp as Police Inspector Lewis Lane

Gale Page as Nurse Randolph

Henry O'Neill as Judge

John Litel as Mr. Monroe, Prosecuting Attorney

Thurston Hall as Grant

Ward Bond as Tug

Ronald Reagan as Radio Announcer (voice) (uncredited)

Mary Field as Millie - Mrs. Updyke's Maid (uncredited)

Maxie Rosenbloom as Butch

Vladimir Sokoloff as Popus

Bert Hanlon as Pat

Curt Bois as Rabbit

Billy Wayne as Candy

Robert Homans as Lt. Johnson

Irving Bacon as Foreman of Jury

John Alban as Guest (uncredited)

Frank Anthony as Gambler in Room 920 (uncredited)

Wade Boteler as Captain MacLevy (uncredited)

Sidney Bracey as Police Chemist (uncredited)

John Breen as Spectator at Robbery (uncredited)

Georgia Caine as Mrs. Frederick R. Updyke (uncredited)

Romaine Callender as Roberts - Updyke's Butler (uncredited)

Glen Cavender as Alarm Company Clerk (uncredited)

Loia Cheaney as Nurse Conner (uncredited)

Bud Cokes as Courtroom Spectator (uncredited)

Hal Craig as Detective Getting Rocks' Fingerprints (uncredited)

Ray Dawe as Policeman (uncredited)

Hal K. Dawson as Pedestrian at Burglary Site (uncredited)

Earl Dwire as Surgeon (uncredited)

Frank Fanning as Fingerprint Expert (uncredited)

J.C. Fowler as Gambler (uncredited)

Edward Gargan as Police Sergeant (uncredited)

William Haade as Mrs. Updyke's Watchman (uncredited)

Winifred Harris as Mrs. Ganswoort - Patient (uncredited)

John Harron as Alarm Company Operator (uncredited)

Thomas E. Jackson as Inspector Connors (uncredited)

Mike Lally as Gambler (uncredited)

Vera Lewis as Juror (uncredited)

Al Lloyd as Sequin Hotel Desk Clerk (uncredited)

Bruce Mitchell as Bailiff (uncredited)

Edmund Mortimer as Guest (uncredited)

Jack Mower as Second Detective (uncredited)

Bob Reeves as Policeman (uncredited)

Frank Reicher as Professor O.J. Ludwig (uncredited)

Ky Robinson as Third Detective (uncredited)

Ruth Robinson as Updyke's Guest (uncredited)

Eric Stanley as Dr. Ames (uncredited)

Larry Steers as Guest (uncredited)

Amzie Strickland as Patient (uncredited)

Libby Taylor as Mrs. Jefferson - Jo's Maid (uncredited)

Arthur Thalasso as Forensic (uncredited)

Monte Vandergrift as First Detective - with Earphones (uncredited)

Joyce Williams as Patricia (uncredited)

Lawrence A. Williams as Juror (uncredited)

William Worthington as Guest (uncredited)