Andrew Garfield as Spider-Man / Peter Parker

Emma Stone as Gwen Stacy

Rhys Ifans as The Lizard / Dr. Curt Connors

Denis Leary as Captain Stacy

Martin Sheen as Uncle Ben

Sally Field as Aunt May

Irrfan Khan as Rajit Ratha

Campbell Scott as Richard Parker

Embeth Davidtz as Mary Parker

Chris Zylka as Flash Thompson

Max Charles as Peter Parker (Age 4)

C. Thomas Howell as Jack's Father

Jake Keiffer as Jack

Kari Coleman as Helen Stacy

Michael Barra as Store Clerk

Leif Gantvoort as Cash Register Thief

Andy Pessoa as Gordon

Hannah Marks as Missy Kallenback

Kelsey Asbille as Hot Girl

Kevin McCorkle as Mr. Cramer

Andy Gladbach as Physics Nerd

Ring Hendricks-Tellefsen as Physics Nerd

Barbara Eve Harris as Miss Ritter

Stan Lee as School Librarian

Danielle Burgio as Nicky's Girlfriend

Tom Waite as Nicky

Keith Campbell as Car Thief

Steve DeCastro as Car Thief Cop

Jill Flint as Receptionist

Mark Daugherty as OsCorp Intern

Milton González as Rodrigo Guevara

Skyler Gisondo as Howard Stacy

Charlie DePew as Philip Stacy

Jacob Rodier as Simon Stacy

Vincent Laresca as Construction Worker

Damien Lemon as Taxi Driver

Ty Upshaw as Police Officer with Sketch

James Chen as Police Officer

Alexander Bedria as Officer (SWAT)

Tia Texada as Sheila (Subway)

Jay Caputo as Subway Guy

John Burke as Newscaster (News Chopper)

Terry Bozeman as Principal

Jennifer Lyons as Second Girl (Subway)

Michael Massee as Man in the Shadows

Amber Stevens West as Ariel

Max Bogner as Scientist (uncredited)

Ethan Cohn as Lab Technician (uncredited)

Miles Elliot as Billy Connors (uncredited)

Miranda LaDawn Hill as Cheerleader (uncredited)

Amanda MacDonald as Goth Girl (uncredited)

Maury Morgan as Tina (uncredited)

Michael Papajohn as Alfie (uncredited)