Tobey Maguire as Spider-Man / Peter Parker

Kirsten Dunst as Mary Jane Watson

James Franco as Harry Osborn

Alfred Molina as Doc Ock / Otto Octavius

Rosemary Harris as May Parker

J.K. Simmons as J. Jonah Jameson

Donna Murphy as Rosalie Octavius

Daniel Gillies as John Jameson

Dylan Baker as Dr. Curt Connors

Bill Nunn as Joseph 'Robbie' Robertson

Vanessa Ferlito as Louise

Aasif Mandvi as Mr. Aziz

Willem Dafoe as Green Goblin / Norman Osborn

Cliff Robertson as Ben Parker

Ted Raimi as Hoffman

Elizabeth Banks as Miss Brant

Bruce Campbell as Snooty Usher

Gregg Edelman as Dr. Davis

Elya Baskin as Mr. Ditkovitch

Mageina Tovah as Ursula

Daniel Dae Kim as Raymond

Hal Sparks as Elevator Passenger

Joel McHale as Mr. Jacks

Stan Lee as Man Dodging Debris

Kelly Connell as Dr. Isaacs

Brent Briscoe as Garbage Man

Emily Deschanel as Receptionist

Jason Fiore-Ortiz as Henry Jackson

Scott Spiegel as Man on Balcony

Andy Bale as OsCorp Executive

Christine Estabrook as Mrs. Jameson

Molly Cheek as Society Woman

John Paxton as Houseman

Joy Bryant as Woman at Web

Joanne Baron as Skeptical Scientist

Peter McRobbie as OsCorp Representative

Timothy Jerome as Injured Scientist

Taylor Gilbert as Mrs. Watson

Peter Vouras as Stage Manager

Donnell Rawlings as Pizza "Heist" Witness

Zachry Rogers as Boy saved by Spider-Man

Ella Rogers as Girl saved by Spider-Man

Louis Lombardi as Poker Player

Marc John Jefferies as Amazed Kid

Roshon Fegan as Amazed Kid

Brendan Patrick Connor as Theater Traffic Cop

Reed Diamond as 'Algernon'

Dan Callahan as 'Jack'

Elyse Dinh as Violinist

John Landis as Doctor

Tim Storms as Chainsaw Doctor

Susie Park as Clawing Nurse

Patricia M. Peters as Screaming Nurse

Michael Edward Thomas as Man at Fire

Anne Betancourt as Woman at Fire

Venus Lam as Child in Burning Building

Bill E. Rogers as Fireman

Joe Virzi as Fireman

Tom Carey as Train Conductor

Jopaul Van Epp as Boy with Mask

Weston Epp as Boy with Mask

Peter Allas as Train Passenger

Brianna Brown as Train Passenger

Bill Calvert as Train Passenger

Tony Campisi as Train Passenger

Joey Diaz as Train Passenger

Chloe Dykstra as Train Passenger

Simone Gordon as Train Passenger

Dan Hicks as Train Passenger

Julia Max as Train Passenger

Savannah Pope as Train Passenger

Timothy Patrick Quill as Train Passenger

Jill Sayre as Train Passenger

Rickey G. Williams as Train Passenger

Michael Arthur as NYPD Officer (uncredited)

Frank Bonsangue as Pizza Man (uncredited)

Cindy Cheung as Chinese Daughter (uncredited)

Phil LaMarr as Train Passenger (uncredited)

Andre M. Johnson as Fireman 2 (uncredited)

Peter Cincotti as Piano Player in Planetarium (uncredited)

Peyton List as Little Girl Playing on Steps (uncredited)

Spencer List as Little Boy Playing on Steps (uncredited)

Troy Metcalf as Blue Collar Guy (uncredited)

Scott Ross as Pedestrian (uncredited)

Bonnie Somerville as Screaming Woman (uncredited)

Wesley Volcy as Columbia University Student (uncredited)

Lou Volpe as Man at Web (uncredited)

Garrett Warren as Bearded Doctor (uncredited)

Joseph M. Caracciolo as Priest at Mary Jane's Wedding (uncredited)

David Boston as Pedestrian (uncredited)

John Cameron as Man Buying 'Spider-Man No More' Newspaper (uncredited)

Sho Brown as Passenger (uncredited)

Sal Ardisi as Parent (uncredited)

Edward Johnson as Planetarium (uncredited)

Grant Curtis as Hot Dog Vendor (uncredited)

Vince Cupone as Victim (uncredited)

William E. Corcoran as Stock Broker (uncredited)

Mohammed Hassan as Middle Eastern Taxi Driver (uncredited)

Rachel Lehrer as Hot Girl (uncredited)

Gene LeBell as Train Passenger (uncredited)

Lorelei Llee as Bank Employee (uncredited)

Joseph Nelson as Extra (uncredited)

Claudia Katz Minnick as Robbie's Assistant (uncredited)

Ronn Ozuk as Cop (uncredited)

Denney Pierce as Train Passenger (uncredited)

Carla Rhodes as Phone Booth Girl (uncredited)

Randy Reinholz as Train Passenger (uncredited)

Rachelle Roderick as Jane, Engineer (uncredited)

Angel Katherine Taormina as Female Onlooker on Street (uncredited)

Douglas Swander as Train Passenger (uncredited)

Jimmy Star as Reporter (uncredited)

Jack Wetherall as Train Passenger (uncredited)

Ken Wharton as Uptight Businessman (uncredited)

Henry Truong as Doc Ock Lab Assistant #2 (uncredited)

Al Collado as Cab Driver (uncredited)

Lee Cogburn as Train Passenger (uncredited)

Sam Raimi as University Student with Bag (uncredited)