Basil Rathbone as Sherlock Holmes

Nigel Bruce as Dr. John H. Watson

Alan Mowbray as Major Duncan-Bleek

Dennis Hoey as Inspector Lestrade

Renee Godfrey as Vivian Vedder

Frederick Worlock as Prof. William Kilbane

Mary Forbes as Lady Margaret Carstairs

Skelton Knaggs as Sands - Moran's Accomplice

Billy Bevan as Conductor Taking Tickets

Geoffrey Steele as Roland Carstairs

Janet Murdoch as Mrs. Shallcross (uncredited)

Gerald Hamer as Alf Shallcross (uncredited)

Leyland Hodgson as Senior Conductor (uncredited)

Harry Cording as Mock the Undertaker (uncredited)

Charles Knight as Baggage Car Guard (uncredited)

Boyd Davis as "Inspector MacDonald" (uncredited)

Bobby Wissler as Mock Jr. (uncredited)

Gilbert Allen as Dining Car Steward (uncredited)

C. Aubrey Smith as Elderly gentleman on train station (uncredited)