Kyla Pratt as Maya Dolittle

Peter Coyote as President Sterling

Malcolm Stewart as Chief Dorian

Niall Matter as Cole Fletcher

Elise Gatien as Courtney Sterling

Karen Holness as Lisa Dolittle

Christine Chatelain as Selma

Kwesi Ameyaw as Prince Tharoor

Stephanie Belding as Doris Park-Weaver

Christopher Gaze as Academic #1

Andrew McIlroy as Academic #2

Kirsten Kilburn as Co-Ed

Adam Thomas as Frat Boy

Jason Griffith as Guard

Alex Kliner as Janitor

Jennifer Coolidge as Daisy (voice)

Richard Kind as Groundhog (voice)

Phil Proctor as Monkey (voice)

Ben Diskin as Anteater (voice)

Greg Ellis as Wallaby (voice)

Nolan North as Parrot (voice)

Diana Yanez as Chinchilla (voice)

Eliza Bayne as Various Talking Animals (voice) (uncredited)

Keith Dallas as Director (uncredited)

Norm Macdonald as Lucky (voice) (uncredited)

Steve Oatway as Mailman (uncredited)