Eddie Murphy as Dr. John Dolittle

Kristen Wilson as Lisa Dolittle

Kyla Pratt as Maya Dolittle

Raven-Symoné as Charisse Dolittle

Steve Zahn as Archie (voice)

Lisa Kudrow as Ava (voice)

James Avery as Eldon

Andy Richter as Eugene Wilson

Lil' Zane as Eric

Denise Dowse as Secretary

Elayn J. Taylor as Eldon's Wife

Kevin Pollak as Riley / Alligator

Victor Raider-Wexler as Judge B. Duff

Jeffrey Jones as Joe Potter

Mark Griffin as Logger / Nature Show Narrator

Ken Hudson Campbell as Animal Control Officer / Dog / Animal Groupie #3 / Forest Animal (voice)

Anne Stedman as Woman

Tommy Bush as Farmer

Adam Vernier as Farm Worker

Chris Marley as Yuppie Man

Shaun Robinson as Newscaster

Googy Gress as Bear Announcer

Lisa Marie Hugueley as Shamu Trainer

Steve Irwin as The Crocodile Hunter

Doug Seus as Klondike Dave Delivery Man

Louise Osbourne as Waitress

Brie Hill Arbaugh as Chihuahua Lady

E.J. Wessel as Guy Throwing Frisbee

Jacob Vargas as Pepito (voice)

Michael Rapaport as Joey the Raccoon (Voice)

Isaac Hayes as Possum (voice)

Andy Dick as Lennie the Weasel (voice)

Joey Lauren Adams as Squirrel (voice)

Maria Arcé as Fish (voice)

Melique Berger as Fish (voice)

David Cross as Dog / Animal Groupie #2 (voice)

David DeLuise as School Fish #2 (voice)

Trevor Denman as Horse Race Announcer (voice)

John DiMaggio as Seeing Eye Dog / Wassup Fish / Mouse (voice)

Georgia Engel as Giraffe (voice)

Mike Epps as Sonny (voice)

Jamie Kennedy as Bandit / Forest Animal / Animal Groupie #1 (voice)

Tom Kenny as Male Tortoise (voice)

Clyde Kusatsu as Bee (voice)

Cedric the Entertainer as Zoo Bear #1 (voice)

David L. Lander as Bird (voice)

Michael McKean as Bird (voice)

Tara Mercurio as Deer (voice)

Mandy Moore as Girl Bear Cub

Frankie Muniz as Boy Bear Cub (voice)

Bob Odenkirk as Animal Groupie #4 / Forest Animal / Dog (voice)

Phil Proctor as Drunk Monkey (voice)

Reni Santoni as Rat #2 (voice)

Richard C. Sarafian as God Beaver (voice)

Hal Sparks as School Fish #1 (voice)

Renée Taylor as Female Tortoise (voice)

John Witherspoon as Zoo Bear #2 (voice)

Keone Young as Bee (voice)

Jodi Essex as Frisbee Thrower in Park (uncredited)

Lawrence Pressman as Governor of California (uncredited)

Norm Macdonald as Lucky (voice)

Crystal the Monkey as Drunk Monkey (uncredited)

Bart the Bear II as Boy Bear Cub

Arnold Schwarzenegger as White Wolf (voice) (archive sound) (uncredited)

John Leguizamo as Rat #2 (voice, uncredited)