Natalie Portman as Lena

Jennifer Jason Leigh as Dr Ventress

Gina Rodriguez as Anya Thorensen

Tessa Thompson as Josie Radek

Tuva Novotny as Cass Sheppard

Oscar Isaac as Kane

Benedict Wong as Lomax

Sonoya Mizuno as Humanoid / Katie (Med Student)

David Gyasi as Daniel

John Schwab as Paramedic

Sammy Hayman as Mayer

Josh Danford as Shelley

Kristen McGarrity as Lena Double

Kumud Pant as Scientist

Honey Holmes as Scientist (uncredited)

Hiten Patel as Scientist (uncredited)

Kola Bokinni as Peyton (uncredited)

Cosmo Jarvis as Special Ops Soldier (uncredited)

Matthew Simpson as Special Ops Soldier (uncredited)