Fredric March as Dr. Henry Jekyll / Mr. Edward Hyde

Miriam Hopkins as Ivy Pearson

Rose Hobart as Muriel Carew

Holmes Herbert as Dr. John Lanyon

Halliwell Hobbes as Brig. Gen. Sir Danvers Carew

Edgar Norton as Poole

Tempe Pigott as Mrs. Hawkins

Leonard Carey as Briggs, Lanyon's Butler (uncredited)

Sam Harris as Party Guest (uncredited)

Boyd Irwin as Police Inspector (uncredited)

Arnold Lucy as Utterson (uncredited)

Murdock MacQuarrie as Dissenting Doctor at Lecture (uncredited)

Eric Mayne as Lanyon's Associate at Lecture (uncredited)

Robert Adair as Ivy's Admirer at Music Hall (uncredited)

Pat Harmon as Music Hall Customer (uncredited)

John Rogers as Music Hall Waiter (uncredited)

G.L. McDonnell as Hobson, Carew's Butler (uncredited)

Douglas Walton as Blonde Student (uncredited)

Harry Adams as Pub Patron (uncredited)

William Begg as Party Guest (uncredited)

Rita Carlyle as Jekyll's Patient (uncredited)

Frank Goddard as Undetermined Role (uncredited)

Bobby Hale as Pub Patron (uncredited)

Tom London as Undetermined Role (uncredited)

William Marion as Party Guest (uncredited)

Eric Wilton as Briggs - Lanyon's Butler (uncredited)