Spencer Tracy as Dr. Henry 'Harry' Jekyll / Mr. Hyde

Ingrid Bergman as Ivy Peterson

Lana Turner as Beatrix 'Bea' Emery

Donald Crisp as Sir Charles Emery

Ian Hunter as Dr. John Lanyon

Barton MacLane as Sam Higgins

C. Aubrey Smith as The Bishop

Peter Godfrey as Poole

Sara Allgood as Mrs. Higgins

Frederick Worlock as Dr. Heath

William Tannen as Intern Fenwick

Frances Robinson as Marcia

Denis Greene as Freddie

Billy Bevan as Mr. Weller

Forrester Harvey as Old Prouty

Lumsden Hare as Colonel Weymouth

Lawrence Grant as Dr. Courtland

John Barclay as Constable

Hillary Brooke as Mrs. Arnold (uncredited)

Alec Craig as Tripped Waiter (uncredited)

Brandon Hurst as Dr. Lanyon's Butler Briggs (uncredited)

Olaf Hytten as Hobson (uncredited)

Colin Kenny as Constable (uncredited)

Doris Lloyd as Mrs. Marley (uncredited)

Aubrey Mather as Inspector (uncredited)

Al Ferguson as Constable (uncredited)

Mary Field as Wife (uncredited)

Frank Hagney as Drunk (uncredited)

Bobby Hale as Cart Driver (uncredited)

Martha Wentworth as Landlady (uncredited)