Jean Dujardin as Lucky Luke

Melvil Poupaud as Jesse James

Sylvie Testud as Calamity Jane

Alexandra Lamy as Belle

Michaël Youn as Billy The Kid

Daniel Prévost as Pat Poker

Chloé Jouannet as Eleanor

Jean-François Balmer as Le gouverneur

André Oumansky as President

Gabriel Corrado as Luke's Father

Pompeyo Audivert as Undertaker

Atilio Pozzobón as Barman

Alberto Laiseca as Dick Digger

Mathias Sandor as Young Luke

Carolina Presno as Luke's Mother

Carlos Kaspar as Hank

Mirta Wons as Mrs. Flimsey

Jorge Noya as Mr. Flimsey

Claudio Weppler as Phil Defer

Horacio Marassi as Colonel Mc Straggle

Daniel Campomenosi as Dr. Doxey

Kazuomi Takagi as Ming Li Fu

Luciano Cáceres as Henchman

Joaquín Berthold as Henchman

Martín Kohan as Henchman

Óscar Kramer as Politician

Daniela Catz as Saloon Girl

Bruno Salomone as Jolly Jumper (voice)