Tara Strong as Twilight Sparkle / Midnight Sparkle (voice)

Rebecca Shoichet as Sunset Shimmer (voice) / Twilight Sparkle (singing voice)

Ashleigh Ball as Rainbow Dash / Applejack / Lyra Heartstrings (voice)

Andrea Libman as Pinkie Pie / Fluttershy / Bon Bon (voice)

Tabitha St. Germain as Rarity / Vice Principal Luna / Muffins (voice)

Cathy Weseluck as Spike (voice)

Enid-Raye Adams as Gloriosa Daisy (voice)

Brian Doe as Timber Spruce (voice)

Nicole Oliver as Principal Celestia (voice)

Vincent Tong as Flash Sentry / Sandalwood (voice)

Brian Drummond as Filthy Rich (voice)

Michael Dobson as Bulk Biceps (voice)

Kathleen Barr as Trixie Lulamoon (voice)

Richard Ian Cox as Snails (voice)

Lee Tockar as Snips (voice)

Shannon Chan-Kent as Pinkie Pie (singing voice)

Kazumi Evans as Rarity (singing voice)

Kelly Metzger as Gloriosa Daisy (singing voice)