Tara Strong as Sci-Twi / Midnight Sparkle / Twilight Sparkle (voice)

Rebecca Shoichet as Sunset Shimmer (voice) / Sci-Twi (singing voice)

Ashleigh Ball as Applejack / Rainbow Dash (voice)

Andrea Libman as Pinkie Pie / Fluttershy / Bon Bon (voice)

Tabitha St. Germain as Rarity / Vice Principal Luna (voice)

Cathy Weseluck as Spike (voice)

Iris Quinn as Principal Abacus Cinch (voice)

Nicole Oliver as Principal Celestia (voice)

Britt McKillip as Dean Cadance (voice)

Vincent Tong as Flash Sentry / Bus Driver (voice)

Andrew Francis as Shining Armor (voice)

Sienna Bohn as Sugarcoat (voice)

Sharon Alexander as Sour Sweet (voice)

Kelly Sheridan as Indigo Zap (voice)

Britt Irvin as Sunny Flare (voice)

Shannon Chan-Kent as Lemon Zest (voice) / Pinkie Pie (singing voice)

Kazumi Evans as Rarity (singing voice)

Lili Beaudoin as Tennis Match (voice)

Diana Kaarina as Mystery Mint (voice)

Ingrid Nilson as Sweet Leaves (voice)

Kaylee Johnston as Background singer (voice uncredited)