Kevin Conroy as Batman (voice)

Melissa Rauch as Harley Quinn (voice)

Paget Brewster as Poison Ivy (voice)

Loren Lester as Nightwing (voice)

Kevin Michael Richardson as Jason Woodrue (voice)

Eric Bauza as Wesley (voice)

Trevor Devall as Bobby Liebowitz (voice)

John DiMaggio as Sarge Steel (voice)

Robin Atkin Downes as Rhino (voice)

Rob Paulsen as Harold Goldblum (voice)

Mindy Sterling as Project Supervisor (voice)

Bruce Timm as Booster Gold (voice)