Bruce Greenwood as Bruce Wayne / Batman (voice)

Jennifer Carpenter as Selina Kyle (voice)

Anthony Stewart Head as Alfred (voice)

Scott Patterson as James Gordon (voice)

John DiMaggio as Chief Bullock / Big Bill Dust / Additional Voices (voice)

Yuri Lowenthal as Harvey Dent (voice)

Grey DeLisle as Sister Leslie / Jason / Additional Voices (voice)

William Salyers as Hugo Strange (voice)

Bob Joles as Mayor Tolliver (voice)

Tara Strong as Marlene (voice)

Kari Wuhrer as Barbara Gordon / Pamela Isley (voice)

Lincoln Melcher as Dickie (voice)

Bruce Timm as Arkham Radio Man (voice)

David Forseth as Cyrus Gold (voice)

Chris Cox as Father Callahan / Additional Voices (voice)