Megalyn Echikunwoke as Mari McCabe / Vixen (voice)

Stephen Amell as Oliver Queen / Green Arrow (voice)

Grant Gustin as Barry Allen / The Flash (voice)

Emily Bett Rickards as Felicity Smoak / Overwatch (voice)

Carlos Valdes as Cisco Ramon (voice)

Victor Garber as Dr. Martin Stein / Firestorm (voice)

Brandon Routh as Ray Palmer / The Atom (voice)

Katie Cassidy as Laurel Lance / Black Canary (voice)

Maria Canals-Barrera as Dr. Vargas (voice)

Trevor Devall as Drake / Jones (voice)

Neil Flynn as Chuck (voice)

Hakeem Kae-Kazim as Eshu (voice)

Brandon Keener as Silk Tie (voice)

Toks Olagundoye as Mari's Mother / News Reporter / Teaching Assistant (voice)

Anika Noni Rose as Kuasa (voice)

Sean Patrick Thomas as Professor Macalester (voice)

Kari Wuhrer as Patty (voice)

Kevin Michael Richardson as D.O.C Guard (voice)

Kimberly Brooks as Young Mari (voice)