Peter Weller as Bruce Wayne / Batman (voice)

Ariel Winter as Carrie Kelley / Robin (voice)

David Selby as Commissioner James Gordon (voice)

Wade Williams as Harvey Dent / Two-Face (voice)

Michael Emerson as Joker (voice)

Dee Bradley Baker as Don / Fat Mutant / Additional Voices (voice)

Maria Canals-Barrera as Commissioner Ellen Yindel (voice)

Paget Brewster as Lana Lang (voice)

Richard Doyle as The Mayor (voice)

Michael Jackson as Alfred Pennyworth (voice)

Yuri Lowenthal as The Son of Batman / Rookie Police Officer (voice)

Michael McKean as Dr. Bartholomew Wolper (voice)

James Patrick Stuart as Murray / Radio Voice (voice)

James Arnold Taylor as Mr. Hudson / Spud (voice)

Bruce Timm as Thomas Wayne / Hydrant Fireman (voice)

Frank Welker as Mayor Stevenson / Weather Man (voice)

Gary Anthony Williams as Mutant Leader / Anchor Bill (voice)

Rob Paulsen as Jeff Adams (voice)

Mark Valley as Clark Kent / Superman (voice)

Robin Atkin Downes as Oliver Queen (voice)

Tress MacNeille as Selina Kyle / Bruno / Old Woman(voice)

Jim Meskimen as President Ronald Reagan (voice)

Conan O'Brien as David Endochrine (voice)

Andy Richter as Frank (voice, uncredited)

Grey DeLisle as Anchor Carla / Female Mutant (voice)

Gary Anthony Sturgis as Silk / Other Spike / Young Mutant (voice)

Carlos Alazraqui as Hernando / TV Host (voice)

Cathy Cavadini as Joanie / Woman with Hot Dog (voice)

Greg Eagles as Mackie / Ben Derrick (voice)

Townsend Coleman as Morrie / Civilian (voice)

Maurice LaMarche as Dr. Willing / News Anchor (voice)

Sam McMurray as Anchor Ted (voice)

Tara Strong as Young Bruce Wayne / Additional Voices (voice)

Andrea Romano as Woman / Police Dispatch / Woman #2 (voice)

Gwendoline Yeo as Lola Chong (voice)

Manny Jacobs as Merkel (voice)

Jim Wise as Femur (voice)

Lex Lang as Rescued Man (voice)

Jason Charles Miller as Axe Fireman (voice)