Peter Weller as Batman / Bruce Wayne (voice)

Ariel Winter as Robin / Carrie Kelley (voice)

David Selby as Commissioner Gordon (voice)

Michael Emerson as Joker (voice)

Mark Valley as Clark Kent / Superman (voice)

Grey DeLisle as Anchor Carla (voice)

Frank Welker as Mayor Stevenson (voice)

Dee Bradley Baker as Don (voice)

Michael McKean as Bartholomew Wolper (voice)

James Patrick Stuart as Murray / Radio Voice (voice)

Maria Canals-Barrera as Ellen Yindel (voice)

Gwendoline Yeo as Lola Chong (voice)

Danny Jacobs as Merkel (voice)

Townsend Coleman as Morrie (voice)

Michael Jackson as Alfred Pennyworth (voice)

Bruce Timm as Hydrant Fireman (voice)

Tress MacNeille as Selina Kyle / Bruno / Old Woman (voice)

Paget Brewster as Lana Lang (voice)

Gary Anthony Williams as Anchor Bill (voice)

Conan O'Brien as David Endocrine (voice)

Robin Atkin Downes as Oliver Queen (voice)

Yuri Lowenthal as Son of Batman (voice)

Greg Eagles as Ben Derrick (voice)

Lex Lang as Rescued Man (voice)

Jason Charles Miller as Axe Fireman (voice)

Andrea Romano as Woman #2 (voice)

Andy Richter as Frank (voice, uncredited)