Koichi Yamadera as Batman / Bruce Wayne (voice)

Wataru Takagi as The Joker (voice)

Rie Kugimiya as Harley Quinn / Harleen Quinzel (voice)

Ai Kakuma as Catwoman / Selina Kyle (voice)

Hochu Otsuka as Alfred Pennyworth (voice)

Daisuke Ono as Nightwing / Dick Grayson (voice)

Akira Ishida as Red Hood / Jason Todd (voice)

Kengo Kawanishi as Red Robin / Tim Drake (voice)

Yuki Kaji as Robin / Damian Wayne (voice)

Junichi Suwabe as Deathstroke / Slade Wilson (voice)

Cho as The Penguin / Oswald Cobblepot (voice)

Takehito Koyasu as Gorilla Grodd (voice)

Toshiyuki Morikawa as Two-Face / Harvey Dent (voice)

Atsuko Tanaka as Poison Ivy / Pamela Isley (voice)

Kenta Miyake as Bane (voice)

Anna Mugiho as Monkichi (voice)

Juri Nagatsuma as Monmi (voice)