Paul Reubens as Pee-wee Herman

Kevin Carlson as Clocky / Conky / Floory (voice)

Laurence Fishburne as Cowboy Curtis

Aaron Fletcher as Santa Claus

Ric Heitzman as Cool Cat / Fish / Flower / Mr. Window (voice)

Suzanne Kent as Mrs. Rene

William Marshall as King of Cartoons

George McGrath as Countess / Fish / Flower / Globey (voice)

S. Epatha Merkerson as Reba

Alison Mork as Chairry / Chicky Baby / Magic Screen (voice)

John Paragon as Jambi / Pterri (voice)

Lynne Marie Stewart as Miss Yvonne

Vic Trevino as Ricardo

Wayne White as Dirty Dog / Fish / Flower / Mr Kite / Randy (voice)

Charlie Tickner as Hans

Annette Funicello as Self

Frankie Avalon as Self

Grace Jones as Self

Dinah Shore as Self

Little Richard as Self

Cher as Self

Magic Johnson as Self

Zsa Zsa Gabor as Self

Whoopi Goldberg as Self

Oprah Winfrey as Self

Joan Rivers as Self

Charo as Self

K.D. Lang as Self

Elena Del Rubio as Self

Milly Del Rubio as Self

Eadie Del Rubio as Self