Troy Baker as Batman / The Joker (voice)

Eric Bauza as Leonardo (voice)

Darren Criss as Raphael (voice)

Kyle Mooney as Michelangelo (voice)

Baron Vaughn as Donatello (voice)

Rachel Bloom as Batgirl (voice)

Ben Giroux as Robin (voice)

Brian George as Alfred Pennyworth (voice)

Jim Meskimen as Commissioner Gordon / Scarecrow (voice)

Tom Kenny as The Penguin (voice)

John DiMaggio as Mr. Freeze (voice)

Tara Strong as Harley Quinn / Poison Ivy (voice)

Carlos Alazraqui as Bane (voice)

Cas Anvar as Ra's al Ghul (voice)

Keith Ferguson as Two-Face / Baxter Stockman (voice)

Andrew Kishino as Shredder (voice)