Troy Baker as Batman / Joker (voice)

Eric Bauza as Leonardo (voice)

Darren Criss as Raphael (voice)

Kyle Mooney as Michelangelo (voice)

Baron Vaughn as Donatello (voice)

Carlos Alazraqui as Bane (voice)

Cas Anvar as Ra's al Ghul (voice)

Rachel Bloom as Batgirl (voice)

John DiMaggio as Mr. Freeze (voice)

Keith Ferguson as Baxter Stockman / Two-Face (voice)

Brian George as Alfred (voice)

Ben Giroux as Robin (voice)

Tom Kenny as Penguin (voice)

Andrew Kishino as Shredder (voice)

Jim Meskimen as Commissioner Gordon / Scarecrow (voice)

Tara Strong as Harley Quinn / Poison Ivy (voice)